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目的:探讨延迟的丙酮酸乙酯(EP)治疗对重症急性胰腺炎(SAP)大鼠血清高迁移率族蛋白1(HMGB1)水平和胰外脏器损伤的影响.方法:96只大鼠随机分为3组,假手术组(Sham组,n=32)、重症急性胰腺炎组(SAP组,n= 32)和丙酮酸乙酯延迟治疗组(EP组,n=32),采用胰胆管逆行灌注人工胆汁的方法复制大鼠SAP模型.EP组建模12,18,30h分别尾静脉注射1次EP溶液30 mg/kg.建模后24和48h处死动物取材,取血清检测HMGB1水平及肝肾功能生化指标,取肺组织用于肺损伤检测.结果:延迟的EP治疗能有效降低SAP大鼠血清HMGB1水平.EP组血清丙氨酸转氨酶(ALT, 446±91 IU/L vs 53±98 IU/L.P<0.01)、天冬氨酸转氨酶(AS T.667±103 IU/L vs 1368±271 IU/L,P<0.01)、尿素氮(BUN,38±4 mg/ dL vs 41±4 mg/dL,P=0.05)和肌酐(Cr,1.2±0.3 mg/dL vs 1.8±0.3 mg/dL,P<0.01)水平以及肺湿/干比(8.22±0.42 vs 9.76±0.45,P<0.01)和组织学评分(7.1±0.7 vs 8.4±1.1,P<0.01)均明显低于SAP组.结论:延迟的EP治疗通过下调血清HMGB1水平减轻SAP大鼠胰外脏器损伤.EP可能是SAP患者抗炎治疗和脏器功能保护的有效选择.
Objective: To investigate the effects of delayed ethyl pyruvate (EP) treatment on serum high mobility group box 1 (HMGB1) and pancreatic organ damage in severe acute pancreatitis (SAP) rats.Methods: Ninety-six rats were randomized The rats in the sham operation group (n = 32), the sham operation group (n = 32), the severe acute pancreatitis group (n = 32 in the SAP group) and the delayed treatment group in the ethyl pyruvate group (n = 32) Retrograde infusion of artificial bile SAP rat model.Ep group modeling 12, 18, 30h after injection of tail vein injection of EP solution 30 mg / kg.After modeling 24 and 48h were sacrificed animals were taken serum levels of HMGB1 and Liver and kidney biochemical indicators, lung tissue were taken for lung injury detection.Results Delayed EP treatment can effectively reduce serum HMGB1 level in SAP rats.Serum alanine aminotransferase (ALT, 446 ± 91 IU / L vs 53 ± (BUN, 38 ± 4 mg / dL vs 41 ± SD), aspartate aminotransferase (ASI), aspartate aminotransferase (ASI) (8.22 ± 0.42 vs 9.76 ± 0.45, P <0.05), and creatinine (Cr, 1.2 ± 0.3 mg / dL vs 1.8 ± 0.3 mg / dL, 0.01) and histological score (7.1 ± 0.7 vs 8.4 ± 1.1, P <0.01) were significantly lower than those in SAP group : EP treatment delayed by downregulating reduce serum levels of HMGB1 SAP rat pancreatic organ damage .EP choice may be effective anti-inflammatory treatment and the protection of organ function in patients with SAP.