目的:建立降脂活血片中葛根素的含量测定方法。方法:采用高效液相色谱法,色谱柱为Shimadzu ODS C_(18)(150 mm×4.6mm,5μm),流动相为甲醇-水(25:75),流速为1.0ml·min~(-1),检测波长为250nm,进样量10μl。结果:葛根素在32～320μg·ml~(-1)浓度范围内峰面积与浓度呈良好的线性关系。回归方程为Y=38.78X-6.73,r=0.9998,平均回收率为100.05%,RSD为1.2%(n=5)。结论:方法简便,准确。可用于控制降脂活血片的质量。 Objective: To establish a method for the determination of puerarin in Jiangzhi Huoxue Tablets. Methods: HPLC was used. The column was Shimadzu ODS C 18 (150 mm×4.6 mm, 5 μm). The mobile phase was methanol-water (25:75). The flow rate was 1.0 ml·min~(-1). ), The detection wavelength was 250 nm, and the injection volume was 10 μl. Results: There was a good linear relationship between peak area and concentration of puerarin in the range of 32-320 μg·ml -1 . The regression equation was Y=38.78X-6.73, r=0.9998, the average recovery was 100.05%, and the RSD was 1.2% (n=5). Conclusion: The method is simple and accurate. Can be used to control the quality of lipid-lowering blood tablets.