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Purpose: To investigate the mechanism of the Ca2 + signaling in cultured human retinal pigment epithelial (RPE) cells with the protein kinase C(PKC) specific inhibitor-hypericin stimulation.Methods:Cultured human RPE cells were analyzed using the fluorescence Ca2+ dye fluo-3 AM and laser scanning confocal microscope(LSCM) after stimulation with 100nM phorbol 12-myristate 13-acetate(PMA) and (or)5 concentrations of hypericin(1, 2, 3, 4 and 5μM).Results: The normal fluorescence in RPE cells was strong and distributed throughout the cells. The nucleus appeared to be more fluorescent than the cytoplasm. After stimulation with PMA alone or 5 concentrations of hypericin, a rapid decrease in flurescence intensity was observed. There was no obvious difference in decreased curve among 5 concentrations. However, after stimulation with a 24 hr preincubation of PMA and 5 concentrations of hypericin, a further decrease was not observed.Conclusion: Fluo-3 AM appears to be a good indicator of the change in Ca2+ occurr
Purpose: To investigate the mechanism of the Ca2 + signaling in cultured human retinal pigment epithelial (RPE) cells with the protein kinase C (PKC) specific inhibitor-hypericin stimulation. Methods: Cultured human RPE cells were analyzed using the fluorescence Ca2 + dye fluo- Results: The normal fluorescence in (3) and laser scanning confocal microscope (LSCM) after stimulation with 100 nM phorbol 12-myristate 13-acetate (PMA) and After stimulation with PMA alone or 5 concentrations of hypericin, a rapid decrease in flurescence intensity was observed. There was no obvious difference in decreased curve among 5 concentrations. However, after stimulation with a 24 hr preincubation of PMA and 5 concentrations of hypericin, a further decrease was not observed. Confluence: Fluo-3 AM appears to be a good indicator of the ch ange in Ca2 + occurr