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目的 检测术前选择性介入动脉化疗对胰腺癌细胞凋亡和细胞增殖的作用 ,探讨区域性化疗抑制胰腺癌生长的分子机制。方法 采用原位末端标记法 (ISEL)对 3 2例术前行介入化疗和未化疗的胰腺癌患者的病理切片进行细胞凋亡指数和增殖指数的检测 ,同时测定细胞凋亡调控基因bcl 2和bax的表达水平。结果 ( 1)术前介入化疗组胰腺癌细胞凋亡率比未介入化疗组明显增高 ,两组的细胞凋亡率分别为 ( 4 6 89± 2 6 46)和 ( 5 67± 2 43 ) (P <0 0 1) ;而细胞增殖指数 (PCNA)在术前介入化疗组与非化疗组之间无显著差异 (P >0 0 5 )。 ( 2 )肿瘤细胞凋亡率与组织类型有关 ,高分化腺癌中的细胞凋亡率比低分化腺癌高 (P <0 0 5 )。 ( 3 )术前介入化疗组bcl 2表达率低于未介入化疗组 ,bax表达率高于未介入化疗组 (P <0 0 5 )。结论 术前选择性的动脉介入化疗能够诱导胰腺细胞凋亡 ,诱导细胞凋亡是抑制胰腺癌细胞的生长重要途径。
Objective To detect the effect of preoperative interventional arterial chemotherapy on pancreatic cancer cell apoptosis and cell proliferation, and to explore the molecular mechanism of regional chemotherapy inhibition of pancreatic cancer growth. Methods In situ end-labeling (ISEL) was used to detect the apoptotic index and proliferative index in 32 patients with pancreatic cancer who underwent chemotherapy before and after chemotherapy. The apoptosis-regulating gene bcl 2 was also determined. Bax expression level. Results (1) The apoptosis rate of pancreatic cancer cells in the preoperative interventional chemotherapy group was significantly higher than that in the non-invasive chemotherapy group. The apoptotic rates in the two groups were (466.9364.6) and (567±243) ( P <0 0 1); while the cell proliferation index (PCNA) was not significantly different between the preoperative interventional chemotherapy group and the non-chemotherapy group (P > 0 05). (2) The apoptotic rate of tumor cells is related to the type of tissue. The rate of apoptosis in well-differentiated adenocarcinoma is higher than that in poorly differentiated adenocarcinoma (P < 0.05). (3) The expression of bcl-2 in the preoperative interventional chemotherapy group was lower than that in the non-invasive chemotherapy group, and the bax expression rate was higher in the preoperative interventional chemotherapy group than in the non-interventional chemotherapy group (P < 0.05). Conclusion Preoperative arterial interventional chemotherapy can induce apoptosis of pancreatic cells. Inducing apoptosis is an important way to inhibit the growth of pancreatic cancer cells.