论文部分内容阅读
为了探究福寿螺消化纤维素的生理机制,本研究采用PCR-DGGE技术分析了福寿螺胃肠内容物的菌群结构。无菌条件下采集福寿螺胃、肠内容物,提取细菌总DNA;以通用引物扩增细菌16S r DNA V3区;DGGE电泳,回收、克隆、测定DGGE优势条带。结果表明,雌、雄福寿螺胃肠菌群结构相同,均为22种细菌;10个明显条带的序列分别与气单胞菌属(Aeromonas sp.)、希瓦氏菌属(Shewanella sp.)、粘质沙雷菌(Serratia marcescens)、肠杆菌属(Enterobacter)、铁单胞菌属(Ferrimonas sp.)和5个不可培养的细菌的16S r DNA V3区序列高度相似。分离到2株细菌,其中1株为腐败希瓦氏菌。
In order to investigate the physiological mechanism of B. lupulus digestive cellulose, PCR-DGGE technique was used to analyze the flora of the gastrointestinal contents of louse. Under sterile conditions, the stomach and intestine of Loxus spirulina were collected and the total bacterial DNA was extracted. The 16S r DNA V3 region of bacteria was amplified by universal primers. DGGE electrophoresis was used to recover and clone the dominant bands of DGGE. The results showed that there were 22 kinds of bacteria in the gastrointestinal flora of the female and male snails. The sequences of 10 obvious bands were respectively related to Aeromonas sp., Shewanella sp. , Serratia marcescens, Enterobacter, Ferrimonas sp., And the 16S r DNA V3 region sequences of 5 uncultured bacteria. Two strains of bacteria were isolated, one of which was Sheanella spp.