本文进行了电镜结合酸性α-醋酸萘酯酶(ANAE)组化方法的研究,改良了国外悬液细胞ANAE超微结构定位技术。悬液细胞用多聚甲醛-戊二醛固定液固定后,孵育于以α-醋酸萘酯为底物的孵育液内,置暗处20℃孵育2小时,以显示ANAE活性。然后制备电镜标本,用2.5％戊二醛预固定, In this paper, electron microscopy combined with the method of acidic α-naphthylacetatease (ANAE) histochemistry was studied, and the ANAE ultrastructural localization technique of foreign suspension cells was improved. The suspension cells were fixed with paraformaldehyde-glutaraldehyde fixing solution, incubated in an a-naphthyl naphthylate as a substrate, and incubated in a dark place at 20°C for 2 hours to show the ANAE activity. Electron microscope specimens were then prepared and pre-fixed with 2.5% glutaraldehyde.