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为选择遗传多样性大的小麦抗、感赤霉病亲本构建作图群体，对４个感病品种（“Ａｌｏｎｄｒａ”、“中国春”、“宁麦３号”、“扬麦３号”）和４个抗病品种（“望水白”、“苏麦３号”、“８９４０３７”、“８９５００４”）进行了ＲＦＬＰ分析。用３０个探针进行检测，其中８个在抗／感组合间侦察到多态性。以望水白／Ａｌｏｎｄｒａ、苏麦３号／Ａｌｏｎｄｒａ、８９４０３７／Ａｌｏｎ－ｄｒａ这３个组合的ＲＦＬＰ频率最高，适合构建分离群体进行抗病基因的定位。现已构建了这３个组合的重组自交系群体，达Ｆ５代，对其多态性检测正在进行中。已检测的９８个探针，在上述３个组合中的多态性频率分别达到３０．６％、２８．６％和２４．５％。还发现探针ＫＳＵＦ１１与ＥｃｏＲＩ、ＨｉｎｄⅢ和ＢａｍＨＩ的酶切片段的杂交图谱上，在抗病品种望水白、苏麦３号及抗病突变体８９４０３７、８９５００４中各有１条谱带；在感病品种中则无此带，推测可能与抗赤霉病有一定的相关性。
In order to select the parents of the resistant and susceptible FHBs with genetic diversity, four susceptible cultivars (Alondra, Chinese Spring, Ningmai 3 and Yangmai 3) And 4 resistant varieties (Wangshui White, Sumai 3, 894037, and 895004). Twenty probes were used for detection, of which 8 detected polymorphisms in the anti-sense combinations. The combination of Wangshui White / Alondra, Sumai 3 / Alondra, 894037 / Alon-dra had the highest RFLP frequency and was suitable for constructing segregation population for the location of disease-resistance genes. Now these three combinations of recombinant inbred lines have been constructed up to F5 generation, and their polymorphism detection is underway. The 98 probes tested showed 30.6%, 28.6% and 24.5% respectively of the above three combinations. Also found that the probe KSUF11 and EcoRI, Hind Ⅲ and BamHI digested fragments of the hybrid map, resistant varieties Wangshui Bai, Sumai 3 and disease-resistant mutant 894037, 895004 each have a band; in the flu There is no such species in the diseased species, which may be related to the resistance to scab.