【摘 要】
：
Objective To construct p IRES2-ZsG reen1/FⅨexpression vector,using the pcDNA/FⅨplasmid containing FⅨcDNA as template,and expressing in HEK-293cells.Methods T
【机 构】
：
Dept Hematol,2nd Hosp, Shanxi Med Univ,
【出 处】
：
China Medical Abstracts(Internal Medicine)
论文部分内容阅读
Objective To construct p IRES2-ZsG reen1/FⅨexpression vector,using the pcDNA/FⅨplasmid containing FⅨcDNA as template,and expressing in HEK-293cells.Methods The total ORF of FⅨgene was amlified from pcDNA/FⅨplasmid,then the amplified fragment was clonded into the p IRES2-ZsG reen1 vector using the
Objective To construct p IRES2-ZsG reen1 / F IX expression vector, using the pcDNA / F IXplasmid containing F IXcDNA as template, and expressing in HEK-293 cells. Methods The total ORF of F IX gene was amlified from pcDNA / F IXplasmid, then the amplified fragment was clonded into the p IRES2-ZsG reen1 vector using the
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