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目的:探讨苦参碱和氧化苦参碱在体外对人正常肝细胞(HL7702)的影响。方法:①采用MTT法检测苦参碱和氧化苦参碱对肝细胞活力的影响;②采用光镜对给药后的细胞形态进行观察;③检测给予苦参碱和氧化苦参碱后,肝细胞培养上清液中的肝功能指标(ALT、AST、ALP、LDH)。结果:①MTT法显示,2.5~5mg/mL的苦参碱对HL7702细胞有明显的抑制作用(P<0.01),5mg/mL的氧化苦参碱能够显著性降低肝细胞活力(P<0.05);②光镜结果显示,给予苦参碱和氧化苦参碱后,细胞出现皱缩,变圆,体积变小,亮度增加,且药物浓度越大,呈现此状态的数目越多,苦参碱对HL7702细胞形态的影响大于氧化苦参碱;③5mg/mL的苦参碱和氧化苦参碱均能明显升高细胞上清液中的AST、ALP、LDH水平(P<0.01或P<0.05),相同剂量苦参碱的上清液中AST、ALP、LDH水平的升高程度大于氧化苦参碱。结论:苦参碱和氧化苦参碱对肝脏可能具有毒性作用,且苦参碱的肝毒性作用大于氧化苦参碱。
Objective: To investigate the effects of matrine and oxymatrine on human normal hepatocytes (HL7702) in vitro. Methods: ①MTT assay was used to detect the effect of matrine and oxymatrine on the viability of hepatocytes; ② The morphological changes of the cells were observed by light microscopy; ③ After matrine and oxymatrine administration, Liver function indicators (ALT, AST, ALP, LDH) in cell culture supernatants. Results: ① MTT assay showed that matrine at 2.5-5 mg / mL significantly inhibited HL7702 cells (P <0.01). Oxymatrine at 5 mg / mL significantly reduced the viability of hepatocytes (P <0.05). ② The results of light microscopy showed that, after administration of matrine and oxymatrine, the cells appeared shrunken, rounded, the volume became smaller, the brightness increased, and the greater the drug concentration, the more the number of this state, the matrine pair HL7702 cell morphology was greater than that of oxymatrine; ③5mg / mL of matrine and oxymatrine significantly increased the level of AST, ALP and LDH in the cell supernatant (P <0.01 or P <0.05) The same dose of matrine in the supernatant AST, ALP, LDH levels increased more than oxymatrine. Conclusion: Matrine and oxymatrine may have toxic effects on the liver, and matrine has more hepatotoxic effects than oxymatrine.