An R2R3 MYB gene,PeMYBL1,was isolated from male inflorescence of Populus×euramericana by homologous cloning combined with in silico cloning techniques.The full length of PeMYBL1 cDNA was 1 094 bp encoding 276 amino acids.The deduced amino acid sequence contained two conserved MYB domains near the N-terminus,a conserved E1 motif and an acidic Ser/Thr rich region toward its C terminus.Phylogenetic analysis revealed that PeMYBL1 was clustered with AtMYB85 from Arabidopsis thaliana,ZmMYBL1 from Zea mays,OsMYB15 from Oryza sativa and ODORANT1 from Petunia hybrida.Furthermore,expression analysis by RT-PCR showed that PeMYBL1 was expressed in root,stem,leaf,male and female infloresences,and abundantly accumulated in male inflorescences.The expression level of PeMYBL1 increased with the development of male inflorescences,indicating that PeMYBL1 is closely related to male flower development.