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目的:探讨神经肽Y(NPY)基因启动子多态性与缺血性脑卒中的相关性。方法:采用聚合酶链反应(PCR)及基因测序技术,对450例缺血性脑卒中患者及423名正常对照者NPY基因启动子-399T/C(rsl6147)基因型及等位基因频率进行检测。Logistic回归分析去除混杂因素影响,分析其与缺血性脑卒中发病的相关性。结果:脑卒中组NPY基因型和等位基因频率与正常对照组比差异有显著统计学意义,特别是在小动脉闭塞性脑卒中类型中。-399C等位基因与缺血性脑卒中存在相关性,是其重要危险因素(OR=2.398,95%CI=1.036～5.553,P=0.041)。结论:NPY基因启动子多态性可能与缺血性脑卒中的发病存在相关,具有-399C等位基因的个体发生缺血性脑卒中的风险可能显著增加。
Objective: To investigate the relationship between NPY gene promoter polymorphism and ischemic stroke. Methods: Polymerase chain reaction (PCR) and gene sequencing techniques were used to detect the genotype and allele frequencies of NPY promoter -399T / C (rsl6147) in 450 ischemic stroke patients and 423 normal controls . Logistic regression analysis to remove the influence of confounding factors, and analyze the correlation with ischemic stroke. RESULTS: There was a statistically significant difference in NPY genotype and allele frequency between the stroke group and the normal control group, especially in the type of arterial occlusive stroke. The -399C allele is associated with ischemic stroke and is an important risk factor (OR = 2.398, 95% CI = 1.036-5.553, P = 0.041). CONCLUSION: NPY gene promoter polymorphism may be associated with the pathogenesis of ischemic stroke. The risk of ischemic stroke may be significantly increased in individuals with -399C allele.