论文部分内容阅读
Through one year hard work,a mutant strain was selected for high production of citric acid which was cultivated in 100 L cylinder reactor.The amount of citric acid was analyzed by high-performance liquid chromatography(HPLC)with the experimental condition:Atlantis C18 column(4.6 mm×150 mm,5μm)was utilized to separate ingredients of fermentation products.Mobile phase was 0.05 mol/L disodi-μm hydrogen phosphate buffer solution(pH2.8),flowing rate for 0.8 mL/min and the iniection volume
Through one year hard work, a mutant strain was selected for high production of citric acid which was cultivated in 100 L cylinder reactor. Amount of citric acid was analyzed by high-performance liquid chromatography (HPLC) with the experimental condition: Atlantis C18 column (4.6 mm × 150 mm, 5 μm) were utilized to separate ingredients of fermentation products. Mobile phase was 0.05 mol / L disodi-μm hydrogen phosphate buffer solution (pH 2.8), flowing rate of 0.8 mL / min and the iniection volume