本文利用荧光标记方法测量了刀豆素A、麦芽凝集素、酵母多糖刺激引起的巨噬细胞膜电位、胞浆pH、溶酶体pH的变化。结果显示三种配体均导致细胞膜电位超极化，胞浆pH降低，溶酶体pH升高，三个生理参量趋于稳定时间稍有不同。胞浆pH的降低可能有抑制内吞的作用，溶酶体pH上升是触发溶酶体内容物外排的基本因素。内吞引起的这些变化是细胞代谢过程中自我调节和保护的表现。 In this paper, macrophages membrane potential, cytoplasm pH, lysosomal pH changes induced by concanavalin A, malt agglutinin and zymosan were measured by fluorescent labeling method. The results showed that all three ligands lead to hyperpolarization of cell membrane potential, decrease of cytosolic pH and increase of lysosomal pH, and the three physiological parameters tend to be slightly different in the stabilization time. The reduction of cytoplasm pH may inhibit endocytosis, and the increase of lysosomal pH is the basic factor that triggers the efflux of lysosomal contents. These changes caused by endocytosis are manifestations of self-regulation and protection during cellular metabolism.