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以一年生辣椒(Capsicum annuum)材料‘V06C1720’为母本,灌木辣椒(C.frutescens)材料‘H101’为父本,建立包含180个单株的种间F2作图群体,应用SSR和SRAP标记技术构建了共278个标记位点的17个连锁群遗传图谱,图谱全长1 282.10 c M,标记平均间距为4.61 c M。利用QTLNetwork 2.0软件和F2、F2:3表型数据,对辣椒15个主要农艺性状进行了QTL分析,在第1、2、3、4、6、7、10、12、13、14、15和16连锁群上共检测到48个加性QTL和11对上位性QTL,可分别解释5.18%~40.33%和4.09%~13.56%的表型变异。变异率大于10.00%的主效加性QTL有33个,占总数的68.75%;来自灌木辣椒‘H101’的增效等位加性QTL位点有29个,占总数的60.42%。株高、主茎高、果长、果径、单果质量、果形和果实辣味等7个性状的9个加性QTL在两个群体中同时被检出,这些在不同环境及不同遗传背景下能够稳定存在的QTL可为辣椒农艺性状分子标记辅助选择育种、QTL精细定位及克隆提供有价值的参考。
The F1 F2 population of 180 individuals was established by using the parent material “Capsicum annuum” V06C1720 as female parent and C.frutescens ’H101’ as male parent. SSR and SRAP markers A total of 278 linkage groups were constructed, including 17282.10 cM in length and 4.61 cM in average. Using QTLNetwork 2.0 and F2, F2: 3 phenotypic data, 15 major agronomic traits of pepper were analyzed by QTL analysis. At the 1st, 2nd, 4th, 6th, 7th, 10th, 12th, 13th, A total of 48 additive QTLs and 11 pairs of epistatic QTLs were detected on 16 linkage groups, which could explain the phenotypic variation of 5.18% -40.33% and 4.09% ~ 13.56% respectively. There were 33 major additive QTLs with the mutation rate of more than 10.00%, accounting for 68.75% of the total. There were 29 QTLs for allelopathic alleles plus 60.42% of the total QTLs from shrub ’H101’. Nine additive QTLs for seven traits including plant height, main stem height, fruit length, fruit diameter, fruit quality, fruit shape and fruit spicy were detected in two populations at the same time. These QTLs were detected in different environments and in different genetic backgrounds The QTLs that could exist stably could provide valuable information for molecular marker assisted selection breeding, QTL fine mapping and cloning.