继研究正常家兔与动脉粥样硬化形成时红细胞钠泵之后,我们又进行了正常人红细胞钠泵活性的研究。 以肝素抗凝收集正常输血员全血,离心去除血浆及白细胞,红细胞用冷的生理盐水洗三次,压积红细胞用等渗介质稀释10倍,制成1∶10红细胞悬液。在混匀下分别加入4只试管中各1ml,离心取沉淀即为100μl红细胞。于1,2管中各加入200微升R_(b2)Co_3 Following the study of normal rabbits and the formation of atherosclerotic red blood cell sodium pump, we conducted a normal human erythrocyte sodium pump activity study. Hematin anticoagulation was used to collect normal blood donor’s whole blood, centrifuged to remove plasma and white blood cells, red blood cells were washed three times with cold physiological saline, and the red blood cells were diluted 10 times with an isotonic medium to make a 1:10 red cell suspension. Add 1 ml each of 4 test tubes under mixing, and centrifuge to obtain 100 μl of red blood cells. Add 200 microliters of R_(b2)Co_3 to each of the 1 and 2 tubes