论文部分内容阅读
应用全基因组DNA甲基化芯片(Illumina Infinium HumanMethylation27 BeadChip)杂交技术以及转录组RNA测序技术,检测了雄激素依赖性前列腺癌(androgen-dependent prostate cancer,ADPC)细胞株LNCaP和雄激素非依赖性前列腺癌(androgen-independent prostate cancer,AIPC)细胞株LNCaP-AI(androgen independent)中的差异甲基化基因。发现与LNCaP细胞株相比,LNCaP-AI细胞株有990个CpG位点表现为高甲基化,涉及855个基因;2 305个CpG位点表现为低甲基化,涉及1 970个基因。结合转录组mRNA表达结果,筛选出6个超甲基化基因:FAM111B、RAB36、PCDH7、COL6A2、IGF1R、GULP1;8个低甲基化基因:EPHA3、TM4SF1、IGFBP5、FAM105A、RASD1、ITPR2、CYP27B1、UBE2E3。这些差异甲基化基因涉及钙离子信号通路、Wnt信号通路等多个信号通路,参与了细胞的基因表达、信号传导、细胞通讯、细胞运动、细胞黏附以及血管生成等功能,为探讨这些差异甲基化基因在前列腺癌雄激素非依赖性转化过程中发挥的作用奠定了基础。
The use of the Illumina Infinium Human Methylation 27 BeadChip hybridization and transcriptase RNA sequencing to detect androgen-dependent prostate cancer (ADPC) cell line LNCaP and androgen-independent prostate cancer (orrogen independent) differentially methylated genes in the androgen-independent prostate cancer (AIPC) cell line LNCaP-AI. It was found that 990 CpG sites in LNCaP-AI cell line showed hypermethylation involving 855 genes compared with LNCaP cell line; 2 305 CpG sites showed hypomethylation involving 1 970 genes. According to the transcriptome mRNA expression results, 6 hypermethylated genes were selected: FAM111B, RAB36, PCDH7, COL6A2, IGF1R and GULP1; 8 hypomethylated genes: EPHA3, TM4SF1, IGFBP5, FAM105A, RASD1, ITPR2 and CYP27B1 , UBE2E3. These differentially methylated genes are involved in multiple signaling pathways, such as calcium ion signaling pathway and Wnt signaling pathway, and are involved in gene expression, signal transduction, cell communication, cell motility, cell adhesion and angiogenesis. To explore these differences Genes in prostate cancer androgen-independent transformation process laid the foundation for the role.