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为探讨慢性乙型肝炎活检组织HBcAg定量检测与细胞凋亡率及凋亡因子Apo2 7表达的相关性。文章将 37例慢性乙型肝炎患者肝活检组织制备成单细胞悬液 ,用小鼠抗人HBcAg单克隆抗体标记后 ,用AnnexinV FITC/PI双染法分辨凋亡细胞及坏死细胞 ,并以凋亡因子Apo2 7标记上述细胞 ,均应用流式细胞术进行了定性定量检测。结果是慢性乙型肝炎患者肝活检组织细胞中 ,存在着不同程度和比例的坏死细胞和凋亡细胞 ,重度慢性乙型肝炎坏死细胞和凋亡细胞比例显著多于轻度慢性乙型肝炎者 (P <0 0 1)。慢性乙型肝炎活检组织HBcAg含量与肝细胞凋亡率、Apo2 7因子表达呈显著相关性 ,Apo2 7表达水平随病损程度的加重而增加 ,有统计学差异。文章提示慢性乙型肝炎活检组织HBcAg含量与肝细胞凋亡率、Apo2 7因子表达呈显著相关性 ,有助于判断病损程度和观察疗效 ,有重要的临床意义
To investigate the relationship between the quantitative detection of HBcAg in chronic hepatitis B biopsies and the apoptosis rate and the expression of Apo2 7. In this paper, 37 patients with chronic hepatitis B liver biopsy tissue prepared single cell suspension with mouse anti-human HBcAg monoclonal antibody labeled with Annexin V FITC / PI double staining of apoptotic cells and necrotic cells, and to withered Apoptosis factor Apo2 7 labeling of the above-mentioned cells, the application of flow cytometry were qualitative and quantitative detection. The result is that there are different degrees and proportions of necrotic cells and apoptotic cells in the liver biopsies of patients with chronic hepatitis B. The proportion of necrotic and apoptotic cells in severe chronic hepatitis B is significantly more than that of mild chronic hepatitis B P <0 0 1). The HBcAg level in chronic hepatitis B biopsy tissue was significantly correlated with hepatocellular apoptosis and Apo2 7 expression, and the expression of Apo2 7 increased with the degree of lesion. There was a significant difference. The article suggests that the HBcAg content in chronic hepatitis B biopsy tissue and liver cell apoptosis rate, Apo2 7 factor expression was significantly correlated, help to determine the extent of lesions and observe the effect, there is an important clinical significance