论文部分内容阅读
未刺激和经大肠杆菌脂多糖(LPS)刺激的腹腔巨噬细胞培养上清液对早期红系祖细胞(BFU-E)增殖均呈促进作用,对晚期红系祖细胞(CFU-E)及粒-单核系祖细胞(CFU-GM)增殖呈双向调控,即低浓度有促进作用,高浓度有抑制作用。其中经LPS刺激的腹腔巨噬细胞培养上清液具有更高的调控活性。这种培养液对多能造血干细胞(CFU-S)的增殖似无明显作用。本研究初步探讨了腹腔巨噬细胞培养上清液的造血调控机理,提示腹腔巨噬细胞培养上清液有促红细胞生成素(EPO)、爆式集落刺激活性(BPA)、粒-单核系集落刺激因子(CSF-GM)样活性,LPS可使巨噬细胞培养上清液中EPO、BPA和CSF-GM样活性增强。
The unstimulated and peritoneal macrophages culture supernatant stimulated by E.coli lipopolysaccharide (LPS) could promote the proliferation of early erythroid progenitor cells (BFU-E), and promote the proliferation of advanced erythroid progenitor cells (CFU-E) and The proliferation of granulocyte-mononuclear stem progenitor cells (CFU-GM) was bidirectional, that is, the promotion of low concentration and the inhibition of high concentration. Among them, LPS-stimulated peritoneal macrophage culture supernatant had higher regulatory activity. This culture medium on pluripotent hematopoietic stem cells (CFU-S) proliferation did not seem to have a significant effect. In this study, the mechanism of hematopoietic regulation of peritoneal macrophage culture supernatant was preliminarily discussed, which suggested that erythropoietin (EPO), blast colony stimulating activity (BPA), granulocyte-monocyte Colony-stimulating factor (CSF-GM) -like activity, LPS enhanced the activity of EPO, BPA and CSF-GM in the macrophage culture supernatant.