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目的:建立高效液相色谱法测定大黄通便颗粒中大黄含量的方法。方法:采用Hypersil XB-C18色谱柱;以甲醇-0.1%磷酸溶液(85:15);检测波长为254nm;理论塔板数按大黄素峰计算应不低于3 000。结果::芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚回归方程的相关系数均在0.999 5以上,平均回收率分别为101.19%、100.86%、101.85%、98.28%、99.65%,RSD分别为2.94%、3.13%、3.33%、1.63%、1.89%。结论:本法精密度好,灵敏度高,可作为大黄通便颗粒质量控制的方法。
Objective: To establish a method for the determination of rhubarb in rhubarb and shigan granules by high performance liquid chromatography. Methods: Hypersil XB-C18 column was used. Methanol-0.1% phosphoric acid solution (85:15) was used. The detection wavelength was 254 nm. The theoretical plate number should be not less than 3000 according to the emodin peak. Results: The correlation coefficients of the regression equations of aloe-emodin, rhein, emodin, chrysophanol and physcion were both above 0.999 5 with the average recoveries of 101.19%, 100.86%, 101.85%, 98.28% and 99.65% , RSD were 2.94%, 3.13%, 3.33%, 1.63%, 1.89% respectively. Conclusion: The method has good precision and high sensitivity, and can be used as a method for quality control of rhubarb constipation granules.