论文部分内容阅读
利用随机扩增DNA多态性分子遗传标记—RAPD ,以杉木J0 和F1 1 两个亲本杂交形成的F1 群体为作图群体 ,从 1 0 4 0个随机引物中筛选出 78个引物 ,对 78个F1 群体及双亲样本进行了RAPD扩增 ,共获得 1 2 9个RAPD标记 ,首次构建了杉木分子遗传连锁框架图 ,其中J0亲本包含 8个连锁群 ,标记覆盖的基因组总长度约为 595.2cM ,F1 1 亲本包含 4个连锁群 ,标记覆盖的基因组总长度约为 31 5.3cM。 1 2 9个RAPD标记中偏分离标记占 1 4.7%。本图谱为构建饱和的杉木分子遗传图谱提供了框架结构 ,为进一步开展杉木分子遗传方面的研究奠定了基础。
Using random amplified polymorphic DNA (RAPD) markers-RAPD, F1 population was crossed with two parents of J0 and F1 1, and 78 primers were screened out from 104 random primers. One F1 population and two parents were RAPD-amplified. A total of 129 RAPD markers were obtained. The first genetic linkage map of Chinese fir was constructed. Among them, J0 parents contained 8 linkage groups, and the total length of the genome covered by the marker was about 595.2 cM , The F1 1 parent contains 4 linkage groups, and the total length of the genome covered by the marker is about 31 5.3 cM. The partial segregation marks of 1 2 9 RAPD markers accounted for 14.7%. This map provides a framework for the construction of saturated Chinese fir molecular genetic map, which lays the foundation for further research on molecular genetic aspects of Chinese fir.