作者应用分离酶(dispase)分离出完整的皮脂腺,并应用3T3细胞饲养层培养技术,成功地培养了来源于皮脂腺的细胞。为进一步研究皮脂腺的形成和功能提供了一个理想的体外培养模型。方法和结果:①皮脂腺的分离:从健康志愿者的胸部或背部取皮肤标本,切成3×5mm小块,放入分离酶(2.4U/ml)4℃20小时后,用细镊分开表皮和真皮。表皮上具有保持完好且连有皮脂腺的毛囊。把表皮放入DNA酶37℃15分钟后,使用显微外科器械在解剖显微镜下从表皮上分离出完整 The authors isolated the complete sebaceous gland by using a dispase and successfully cultured the cells derived from the sebaceous gland by using the 3T3 cell feeder culture technique. It provides an ideal in vitro culture model for further study on the formation and function of sebaceous glands. Methods and Results ① Sebaceous gland separation: The skin specimens were taken from the chest or back of healthy volunteers, cut into 3 × 5mm pieces and placed in separate enzyme (2.4U / ml) for 20 hours at 4 ℃. And leather. The epidermis has hair follicles that remain intact with sebaceous glands. After placing the epidermis in DNase for 15 minutes at 37 ° C, the whole was separated from the epidermis using a microsurgery instrument under a dissecting microscope