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采用甲基磺酸乙酯（ＥＭＳ）诱变草鱼吻端组织细胞（ＺＣ－７９０１）．用乌苯苷（Ｏｕａｂａｉｎ）筛选抗药性突变体．结果表明，ＥＭＳ对草鱼细胞的诱变效果与该药物对哺乳类细胞的诱变效果有明显差异．草鱼细胞对乌苯苷的敏感性也大大低于哺乳类细胞．ＺＣ－７９０１细胞在传代后２４ｈ，用浓度为２．８ｍｇ／ｍｌ的ＥＭＳ处理２４ｈ，诱变效果较理想，细胞杀伤率约３０％，这比通常用ＥＭＳ诱变哺乳类细胞所使用的浓度（０．４ｍｇ／ｍｌ）增大了７倍，存留下来的细胞用浓度为２５ｍｍｏｌ／Ｌ乌苯苷筛选抗性突变体，有少数细胞存活，并具有分裂增殖能力．但在细胞瓶内不易形成单层
Grass carp kisses tissue cells (ZC-7901) were mutagenized by ethyl methanesulfonate (EMS). Drug resistant mutants were screened with Ouabain. The results showed that EMS mutagenic effect of grass carp cells with the drug mutagenic effect of mammalian cells were significantly different. The sensitivity of grass carp cells to eupryxins was also significantly lower than that of mammalian cells. ZM-7901 cells were treated with EMS at a concentration of 2.8 mg / ml for 24 h after passage at 24 h after passage. The mutagenic effect of ZC-7901 cells was better than that of ZC-7901 cells. The killing rate of ZC-7901 cells was about 30% 0.4mg / ml) increased seven-fold, the remaining cells with a concentration of 25mmol / L olanipine screening resistant mutants, a small number of cells survive, and have the ability to divide and proliferate. However, the formation of a single layer in the bottle is not easy