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目的观察氯己定乙醇与碘伏对医院分离的26株耐甲氧西林金黄色葡萄球菌(MRSA)和标准菌株金黄色葡萄球菌(ATCC 29213)的体外抗菌活性。方法采用经典二倍稀释法,进行最低抑菌浓度(MIC)试验和最低杀菌浓度(MBC)试验。结果两种消毒剂对26株MRSA的MIC值均等于标准菌株(分别为1 mg/L醋酸氯己定乙醇、21 mg/L碘伏),两种消毒剂对其中23株MRSA的MBC值等同于MIC值。醋酸氯己定乙醇对2株MRSA的MBC值为标准菌株的31倍;碘伏对2株MRSA的MBC值为标准菌株的2倍;其中1株MRSA对醋酸氯己定乙醇的MBC值为标准菌株的31倍,同时对碘伏的MBC值为标准菌株的2倍。结论对醋酸氯己定乙醇及碘伏均产生抗性的MRSA菌株已经出现,且具有交叉耐药性。应积极探讨高效低毒消毒剂的研制与合理应用、消除细菌对消毒剂抗性的问题。
Objective To observe the in vitro antibacterial activity of 26 methicillin-resistant Staphylococcus aureus (MRSA) and standard strain Staphylococcus aureus (ATCC 29213) isolated from hospital-isolated chlorhexidine ethanol and iodophor. Methods The classical two-fold dilution method was used to test the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Results The MIC of 26 MRSA strains was equal to that of the standard strains (1 mg / L chlorhexidine acetate and 21 mg / L iodophor respectively). The MBC values of the 23 strains of MRSA were the same as that of the two disinfectants At MIC value. The MBC value of chlorhexidine acetate acetate against two MRSA strains was 31 times that of the standard strain. The MBC value of two strains of MRSA against iodophor was twice as high as that of the standard strain. Among them, MBC value of one MRSA against chlorhexidine acetate was the standard 31 times the strain, MBP value of iodine at the same time as the standard strain of 2 times. Conclusion MRSA strains that are resistant to both chlorhexidine acetate and iodophor have emerged and are cross-resistant. Should actively explore the development of high efficiency and low toxicity disinfectant and rational application of bacteria to eliminate the problem of resistance to disinfectants.