论文部分内容阅读
利用单克隆抗体技术 ,从抗顶体反应后精子头的单克隆抗体库中 ,筛选出一株单抗MSH2 7.在顶体反应后 ,抗原定位于精子与卵子质膜融合的起始位置———精子的赤道段和顶体后区 ;此抗体能够阻断精卵膜融合 ,但不影响精子与卵子质膜的结合 .这种阻断作用呈现抗体剂量依赖性 ,用含 6 0 0 μg/mLIgM型单抗MSH2 7处理精子 ,受精指数下降 90 % .免疫亲合层析分离纯化的抗原 (分子量 39ku)作用于卵子质膜 ,也可使精卵膜融合指数明显地降低 .这些结果说明MSH2 7抗原分子在精卵膜融合过程中起着重要作用
Monoclonal antibody technique was used to screen out a monoclonal antibody MSH2 from the sperm head monoclonal antibody library after anti-acrosome reaction. 7. After the acrosome reaction, the antigen was located at the start of the fusion of sperm and egg plasma membrane - - the equatorial part of the sperm and the apocrine region; this antibody blocks the fusion of the spermatozoa but does not affect the binding of the sperm to the egg membrane. This blocking effect appears in a dose-dependent manner with antibodies containing 6 0 0 μg / mL IgM monoclonal antibody MSH2 7, the fertilization index decreased by 90% Immunoaffinity chromatography purified antigen (molecular weight 39ku) on the oocyte plasma membrane, but also can make the egg membrane fusion index decreased significantly.These results indicate MSH2 7 antigen molecules play an important role in the process of eosinophilic integration