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目的探讨宫颈鳞状细胞癌组织中人乳头瘤病毒(HPV)感染基因型与致癌性的关系。方法来自一项多中心的临床研究的宫颈鳞状细胞癌组织标本653例,使用膜杂交芯片技术行23种HPV分型检测,并对患者的相关资料进行分析。结果宫颈鳞状细胞癌组织标本中,总的HPV感染率为90.05%(588/653),其中一型感染率为73.97%(483/653),多型感染率为16.08%(105/653)。宫颈鳞状细胞癌组织中,HPV一型感染是主流,多型感染是支流,HPV感染最主要的高风险型别依次为16、18、58、33、31、52、59、45、66、73型,16型最多,占高危型出现频率的60.90%(422/693),16型和18型占高危型出现频率的71.00%(492/693)。结论 HPV基因膜芯片检测技术可用于宫颈鳞状细胞癌组织的分型检测,对我国女性宫颈鳞状细胞癌的防治、HPV疫苗的研发以及宫颈鳞状细胞癌组织HPV感染分型数据库的建立都具有重要的意义。
Objective To investigate the relationship between human papillomavirus (HPV) genotype and carcinogenicity in cervical squamous cell carcinoma. METHODS: A total of 653 cervical squamous cell carcinoma specimens from a multicenter clinical trial were screened for 23 HPV types using the membrane hybridization technique, and the patient-related data were analyzed. Results The total HPV infection rate in cervical squamous cell carcinoma was 90.05% (588/653), of which the infection rate of type 1 was 73.97% (483/653) and the rate of polytype infection was 16.08% (105/653) . Cervical squamous cell carcinoma, HPV type one infection is the mainstream, multi-type infection is tributary, HPV infection is the most important high-risk type followed by 16,18,58,33,31,52,59,45,66, Type 73 and Type 16 accounted for the largest, accounting for 60.90% (422/693) of the high-frequency occurrences, while Type 16 and 18 accounted for 71.00% (492/693) of the high-frequency occurrences. Conclusion HPV gene chip detection technology can be used for the detection of cervical squamous cell carcinoma tissue typing, prevention and treatment of cervical cancer in our country, HPV vaccine research and development of cervical squamous cell carcinoma tissue HPV infection typing database It is of great significance.