论文部分内容阅读
采刚体外大鼠红细胞溶血实验和小鼠腹腔巨噬细胞体外培养的方法,研究了硫酸锌与石英尘对大鼠红细咆、小鼠腹腔巨噬细胞的联合作用。结果表明,硫酸锌能明显拮抗体外石英尘耐细胞的毒性作用,表现为染尘后同一时间硫酸锌与石英尘联合作用组和单纯石英尘作用组相比,红细胞溶血百分率降低(P<0.05);巨噬细胞成活率明显提高(P<0.05);巨噬细胞胞浆内乳酸脱氢酶(LDH)漏出量降低(P<0.05)。推测其拮抗作用机理可能为Zn~(+2)与石英尘表面离解的硅烷醇基团相结合,阻隔了该丛团对细胞的作用,从而降低了石英尘的毒作用。
To investigate the combined effect of zinc sulfate and quartz dust on rat erythrocyte hemolysis and mouse peritoneal macrophages in vitro using erythrocyte hemolysis assay and in vitro culture of murine peritoneal macrophages. The results showed that zinc sulfate could obviously antagonize the toxic effect of quartz dust-tolerant cells in vitro. The results showed that the percentage of hemolysis of erythrocytes was decreased (P <0.05) at the same time when zinc sulfate and quartz dust were combined with pure quartz dust at the same time, ; The survival rate of macrophages was significantly increased (P <0.05); the leakage of lactate dehydrogenase (LDH) in macrophages was decreased (P <0.05). It is speculated that the mechanism of antagonism may be Zn ~ (+2) combined with silanol groups dissociated on the surface of quartz dust, blocking the effect of the clumps on cells and thus reducing the toxic effect of quartz dust.