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目的:优化广藿香挥发油的提取方法并考察其抗肿瘤活性。方法:在蒸馏法提取广藿香油之前进行酶解处理,通过正交试验研究酶的用量、酶解时间、温度以及pH对广藿香油提取率的影响,优选最佳工艺。采用气-质联用(GC-MS)测定广藿香挥发油的组成以及各成分的相对百分含量。通过MTT法测定广藿香油对Hela细胞增殖活性的抑制作用。结果:酶辅助提取广藿香油的最佳条件是:酶用量为生药重量的1%,在pH 4.5,45℃条件下酶解1 h;药典法、酶解法提取的总挥发油的提取率分别为2.2220、3.1360 mg/g;两种方法提取的广藿香油抑制He-la增殖细胞活性的IC50分别为:12.2±0.46、0.36±0.03μg/mL。结论:酶解法可提高广藿香挥发油的提取率,该法提取的广藿香油抑制Hela细胞增殖的活性强于药典法提取的广藿香油。
Objective: To optimize the extraction method of volatile oil from Pogostemon cablin and study its antitumor activity. Methods: Before the extraction of patchouli oil by distillation, enzymatic hydrolysis was carried out. The effects of enzyme dosage, enzymolysis time, temperature and pH on the extraction rate of patchouli oil were studied by orthogonal test, and the optimum process was optimized. Gas chromatography-mass spectrometry (GC-MS) was used to determine the composition of volatile oil of Pogostemon cablin and the relative percentage of each component. Inhibition of patchouli oil on Hela cell proliferation by MTT assay. Results: The optimal conditions for the enzyme-assisted patchouli oil extraction were as follows: the enzyme dosage was 1% of the weight of the crude drug and enzymolysised at pH 4.5 and 45 ℃ for 1 h; the extraction rates of total volatile oil extracted by the pharmacopoeia method and enzymolysis method were 2.2220 and 3.1360 mg / g, respectively. The IC50 of the patchouli oil extracted from the two methods for inhibiting proliferation of He-la cells were 12.2 ± 0.46,0.36 ± 0.03 μg / mL, respectively. Conclusion: The enzymolysis method can improve the extraction rate of volatile oil from patchouli. The activity of patchouli oil extracted from Hela cells inhibited by Hela cells was stronger than that of patchouli oil extracted from Pharmacopoeia.