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通过拨动细胞实现遗传物质的移动,并在对卵母细胞的拨动机理进行深入分析的基础上,根据极体是否可见将卵母细胞的自动拨动过程分为翻动和转动两部分。进一步的,对翻动过程中的卵母细胞进行建模,并通过实验首次提出并实现了四种卵母细胞的自动拨动轨迹。随后,对影响卵母细胞翻动幅度的因素进行了研究,并通过实验证实翻动的幅度可以通过拨动微针的离焦高度进行控制。最后,绵羊的大批量核移植操作应用结果表明,使用设计的自动拨动轨迹的移动遗传物质的成功率可达95%以上。同时,拨动过程中极体出现的位置也和本文提出的翻动模型计算的结果一致,从而验证了本文工作的有效性。
Based on the in-depth analysis of the toggle mechanism of oocytes, the automatic dialing process of oocytes is divided into turning and rotating according to whether the polar body is visible or not. Furthermore, the oocytes in turning process were modeled, and the automatic dialing trajectories of four kinds of oocytes were proposed and realized for the first time. Subsequently, the impact on the oocyte turnover rate of the factors were studied and confirmed by experiments that the magnitude of flip can be controlled by turning the microneedle defocus height. Finally, the results of the application of high-volume nuclear transfer in sheep show that the success rate of moving genetic material using the designed autodotative trajectory can reach more than 95%. At the same time, the position of the polar body in the toggle process is also consistent with the result of the flipping model proposed in this paper, which verifies the validity of the work in this paper.