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目的建立连翘药材HPLC指纹图谱。方法采用HPLC法,用Shiseido Capcell pak MG C18色谱柱(250mm×4.6mm,5μm),乙腈-0.2%磷酸梯度洗脱,流速为1ml.min-1,检测波长210nm,柱温:25℃。以中药色谱指纹图谱相似度评级系统(版本2004A)软件计算,先建立标准指纹图谱,并与其他产地翘药相似度计算。结果以河南卢氏产连翘建立标准指纹图谱,其他产地连翘药材指纹图谱的相似度在0.704-0.938,确定了12个共有峰,建立了连翘药材HPLC指纹图谱。结论本法可作为连翘药材的质量控制方法。
Objective To establish HPLC fingerprints of Forsythia suspensa. Methods The HPLC method was performed on a Shiseido Capcell pak MG C18 column (250 mm × 4.6 mm, 5 μm) with a gradient of acetonitrile-0.2% phosphoric acid. The flow rate was 1 ml · min-1. The detection wavelength was 210 nm and the column temperature was 25 ℃. To Chinese medicine chromatographic fingerprint similarity rating system (version 2004A) software to calculate, first establish a standard fingerprinting, and with other origin warfarin similarity calculation. Results The standard fingerprints of forsythia from Henan were established. The similarity of fingerprints of forsythia from other fields was 0.704-0.938, and 12 common peaks were identified. HPLC fingerprints of forsythia was established. Conclusion This method can be used as the quality control method of Forsythia.