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观察水蛭素对凝血酶造成的血管内皮细胞单层通透性增高的抑制作用。从新生牛主动脉分离获得单个内皮细胞 ,培养于微孔滤膜上直至形成致密单层。通过灌流Hanks液或含 5g·L-1 白蛋白的Hanks液后 ,测定经 1 0U/ml凝血酶溶液处理或经 1 0U/ml凝血酶溶液加 1 0U/ml水蛭素处理的液体滤过系数 (Kf)、液体滤过流量(Jv)和蛋白质渗透压反射系数(σ)。结果 :内皮单层经凝血酶处理后 ,Kf和Jv降低 ,σ升高 ;而水蛭素能够抑制因凝血酶造成的Kf和Jv降低 ,以及σ升高。提示 :凝血酶能够引起血管内皮单层通透性的增加 ,而水蛭素能够减轻凝血酶造成的内皮单层通透性的增加 ,其作用机制尚需进一步研究
Observe the inhibitory effect of hirudin on thrombin-induced increase in vascular endothelial monolayer permeability. Single endothelial cells were isolated from newborn bovine aorta and cultured on microporous filters until a dense monolayer was formed. After perfusion of Hanks solution or Hanks solution containing 5 g·L-1 albumin, the liquid filtration coefficient treated with 10 U/ml thrombin solution or treated with 10 U/ml thrombin solution plus 10 μl/ml hirudin was determined. (Kf), liquid filtration flux (Jv) and protein osmotic pressure reflection coefficient (σ). RESULTS: Endothelial monolayers were treated with thrombin, Kf and Jv decreased, and σ increased. Hirudin inhibited the decrease of Kf and Jv due to thrombin, and σ increased. Tip: Thrombin can increase the permeability of vascular endothelial monolayer, while hirudin can reduce the increase of thrombin-induced endothelial monolayer permeability. The mechanism of action still needs further study.