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采用放射配体结合实验，离体左心房收缩功能实验及逆转录聚合酶链反应等方法观察大鼠长期饮用非选择性β肾上腺素受体拮抗剂普萘洛尔（７０ｍｇ·ｋｇ－１·ｄ－１，８ｗｋ）对心脏α１肾上腺素受体（α１－ＡＲ）亚型分布及其功能的影响．结果表明大鼠饮用普萘洛尔８ｗｋ后心脏α１－ＡＲ总数量无显著变化，ＷＢ４１０１和尼古地平竞争抑制曲线两位点分析结果显示３种亚型受体所占比例发生了明显变化，α１Ａ－ＡＲ数量不变，α１Ｂ－ＡＲ数量减少，α１Ｄ－ＡＲ数量增加，α１－ＡＲ亚型ｍＲＮＡ水平的改变为α１Ａ－ＡＲ和α１Ｄ－ＡＲ显著增加，α１Ｂ－ＡＲ明显减少．但α１－ＡＲ３种亚型介导去甲肾上腺素正性变力效应的作用无显著改变．
Radioligand binding assay, isolated left atrial systolic function test and reverse transcription polymerase chain reaction were used to observe the long-term drinking of propranolol (70 mg · kg-1 · d -1, 8wk) on the distribution and function of cardiac α1-adrenoceptor (α1-AR) subtypes. The results showed that there was no significant change in the total amount of α1-AR in the heart of rats after drinking propranolol for 8 weeks. The results of competitive inhibition curve of WB4101 and nicotine showed that the proportions of the three subtypes of receptors changed obviously, α1A -AR, the number of α1B-AR decreased and the number of α1D-AR increased. The changes of α1-AR subtypes mRNA levels were significantly increased for α1A-AR and α1D-AR, and α1B-AR was significantly decreased. However, there was no significant change in the role of α1-AR3 subtypes in mediating normoxeneic effects of norepinephrine.