论文部分内容阅读
用马铃薯X病毒(PVX)免疫的BALB/c小鼠的脾细胞与骨髓瘤细胞(SP2/0)融合,经3次克隆化后,筛选出3类分泌抗PVX株系特异性的单克隆抗体(McAb)杂交瘤细胞株,该细胞株经传代20代以上,并经液氮冻存一年以后仍表现稳定。3个杂交瘤细胞株染色体数目集中在92—102条之间,其免疫球蛋白亚类均为IgG3,用ELISA间接法测定细胞培养上清滴度为1∶320—1∶640,小鼠腹水抗体滴度为1∶102400—1∶204800,后者比兔抗血清滴度(1∶320)高300倍以上。McAb对抗原具有中和作用,中和滴度为1∶10~2。经反复冻融、硫铵沉淀和冻干后,抗体活性无明显变化。
The spleen cells of BALB / c mice immunized with potato X virus (PVX) were fused with myeloma cells (SP2 / 0). After three clones, three types of monoclonal antibodies secreting anti-PVX strains were screened (McAb) hybridoma cell line, the cell line after more than 20 passages, and after a year of liquid nitrogen frozen still stable. The chromosome numbers of the three hybridoma cell lines concentrated between 92-102, the immunoglobulin subclasses were all IgG3, and the cell culture supernatant titer was determined to be 1: 320-1: 640 by ELISA indirect method. The mouse ascites Antibody titers ranged from 1:102400 to 1:48800, which was more than 300 times higher than rabbit antiserum titers (1: 320). McAb has a neutralizing effect on the antigen, and the neutralization titer is 1:10 ~ 2. After repeated freeze-thaw, ammonium sulfate precipitation and freeze-dried, antibody activity no significant change.