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本文主要研究了不同量的磷酸钙在生长环境下对骨肉瘤细胞死亡的影响。通过在无钙培养基中加入氯化钙原位合成得到所需磷酸钙颗粒,然后用无钙磷培养基按照不同的比例稀释所得磷酸钙颗粒。MTT结果表明,24 h后的细胞凋亡与培养基中磷酸钙的量有关。当浓度超过240μg.mL-1时,细胞出现大量死亡。细胞超薄切片结果也表明,当磷酸钙浓度超过240μg.mL-1时,可以观察到有大量磷酸钙存在于细胞内;而磷酸钙浓度低于240μg.mL-1时,细胞内观察不到磷酸钙的存在。这些结果表明:240μg.mL-1对于细胞死亡是一个非常重要的转折点,可能超出了细胞的自我修复范围或者承受能力。这些结果对于磷酸钙在生物医学工程,如组织工程、药物输送、基因转染等方面具有一定的指导意义。
In this paper, the effects of different dosages of calcium phosphate on osteosarcoma cell death in a growing environment were studied. The desired calcium phosphate particles were obtained by in situ synthesis by adding calcium chloride in a calcium-free medium, and then the resulting calcium phosphate particles were diluted in different proportions with a calcium-free phosphorus-containing medium. MTT results showed that after 24 h apoptosis and the amount of calcium phosphate in the medium. When the concentration exceeds 240μg.mL-1, a large number of cell death occurred. The result of ultrathin section also showed that a large amount of calcium phosphate was observed in the cells when the concentration of calcium phosphate was more than 240μg.mL-1, but not in the cells when the concentration of calcium phosphate was less than 240μg.mL-1 The presence of calcium phosphate. These results indicate that 240 μg.mL-1 is a very important turning point for cell death and may be beyond the cell’s self-healing range or affordability. These results have some guiding significance for calcium phosphate in biomedical engineering, such as tissue engineering, drug delivery, gene transfection and so on.