本文采用单克隆抗体的半定量斑点法测定44株变形链球菌临床分离株合成葡糖基转移酶(glucosyltransferases,GTFs)的种类与含量。 研究对象是1.5～3岁的12名儿童和他们的母亲,进行配对研究。其中6名儿童为龋活跃性,患奶瓶龋,另6名儿童无龋。收集该12名儿童牙面菌斑,石蜡刺激法收集配对母亲的全唾液。MSB琼脂培养后进行菌株血清型和核糖类型鉴定。选择44株菌作GTFs分析。其中血清型c,e,g菌株分别为27株、9株和8株。 In this paper, the use of monoclonal antibodies semi-quantitative dot assay 44 Streptococcus mutans clinical isolates of glucosyltransferases (glucosyltransferases, GTFs) species and content. The study population consisted of 12 children 1.5 to 3 years old and their mothers. Six of the children were active caries, with bottle caries, and six children without caries. The 12 children were collected for dental plaque and the paraffin-stimulated method was used to collect the whole saliva of matched mates. The MSB agar was cultured to identify serotypes and ribotypes. Forty-four strains were selected for GTFs analysis. The serotype c, e, g strains were 27 strains, 9 strains and 8 strains.