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为了探索应用昆虫病原线虫防治二点委夜蛾Athetis lepigone的可行性,在室内比较了6种昆虫病原线虫对二点委夜蛾幼虫的致病力影响,生测结果表明,Heterorhabditis bacteriophora HB8对二点委夜蛾幼虫致病力最高,处理120h后二点委夜蛾5龄幼虫的死亡率为93.33%。本论文还比较了二点委夜蛾不同虫态对H.bacteriophora HB8的敏感性,测定了不同温度、土壤湿度以及不同线虫剂量对H.bacteriophora HB8致病力的影响,结果表明,二点委夜蛾5龄幼虫对H.bacteriophora HB8最敏感,其次是3龄幼虫,而结茧的预蛹和蛹不敏感;在测定范围内,在25～30℃、15%土壤湿度以及侵染期线虫与二点委夜蛾5龄幼虫数量比不少于50∶1的条件下,H.bacteriophora HB8对二点委夜蛾幼虫的致病力最强。
In order to explore the feasibility of controlling Athetis lepigone by using the entomopathogenic nematode, six pathogenic nematodes were compared indoors to determine their pathogenicity. The results of biopsy showed that Heterorhabditis bacteriophora HB8 The larvae of P. exigua had the highest pathogenicity, and the mortality of the 5th instar larvae of S. exigua was 93.33% after 120 h of treatment. In this paper, we compared the susceptibility of H.bacteriophora HB8 to different stages of H. armigera and determined the pathogenicity of H.bacteriophora HB8 at different temperatures, soil moisture and different nematodes doses. The results showed that the two-point committee The fifth instar larvae of H. armigera were the most sensitive to H. bacteriophora HB8, followed by the third instar larvae, while the pre-pupa and pupae of the cocoon were insensitive. Within the range of 25-30 ℃, 15% soil moisture and nematode With the second instar fifth instar larvae number ratio of not less than 50: 1, H.bacteriophora HB8 on the 2nd instar the larvae of the pathogenicity of the strongest.