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目的探讨离心涂片法、夹层杯法和改良罗氏培养法3种方法检测脑脊液中结核分枝杆菌对于结核性脑膜炎的诊断价值。方法对临床确诊的结核性脑膜炎患者、高度可疑结核性脑膜炎患者共50例(结脑组)和30例非结核性脑膜炎患者(对照组)的脑脊液标本采用离心涂片法、夹层杯法和改良罗氏培养法进行检测,对其结果进行对比分析。结果结脑组标本中,离心涂片法、夹层杯法和改良罗氏培养法阳性率分别为1.79%(1/56)、8.93%(5/56)和21.4%(12/56),对照组均未检测到结核菌,比较结脑组3种方法的阳性检测率,其差异有统计学意义(P<0.05)。结论培养法阳性率最高,但其检测时间长,不利于对结脑进行及时诊断,夹层杯法较离心涂片法阳性率高,有条件的医院可适当推广。
Objective To investigate the diagnostic value of Mycobacterium tuberculosis in cerebrospinal fluid (CSF) for tuberculous meningitis by three methods: centrifugation smear method, sandwich method and modified Roche culture method. Methods Fifty patients (tuberculous meningitis group) and 30 non-tuberculous meningitis patients (control group) with clinically confirmed tuberculous meningitis were enrolled in this study. Cerebrospinal fluid (CSF) samples were collected by centrifugation, Law and improved Roche culture method for testing, the results were compared. Results The positive rates of spinach, sandwich cup and modified Roche were 1.79% (1/56), 8.93% (5/56) and 21.4% (12/56) respectively in control group No detection of tuberculosis, compared with three groups of tuberculous meningitis positive detection rate, the difference was statistically significant (P <0.05). Conclusion The culture method has the highest positive rate, but the detection time is long, which is not conducive to timely diagnosis of tuberculous meningitis. The sandwich cup method has the higher positive rate than the centrifugal smear method. The qualified hospitals can be appropriately popularized.