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考察赤牡妇炎胶囊中黄藤药材的提取纯化工艺的方法,优选最佳的提取纯化工艺条件。以盐酸巴马汀提取率为指标,采用单因素试验与正交试验优选黄藤提取工艺,以盐酸巴马汀提取率为指标,采用碱沉盐析法优选黄腾纯化工艺。黄藤最佳提取工艺为:加6倍量0.3%硫酸溶液浸泡提取3次,每次24 h,将提取液调pH至12,加氯化钠适量至盐析浓度为10%,静置24 h;最佳纯化工艺为:将黄藤粗提物粉碎,加30倍85%乙醇回流提取30 min,滤过,回收乙醇至15倍量,静置48 h,滤过,滤渣干燥即得。采用此工艺可达到黄藤药材提取纯化的目的,该方法操作简单,为其工业化生产应用奠定了科学的基础。
To study the method of extracting and purifying the rhynchophylla medicinal materials in the Kumu Fuyan Capsule, the optimal extraction and purification process conditions were optimized. The extraction rate of palmatine hydrochloride was used as an index. The single-factor test and orthogonal test were used to optimize the extraction technology of Paeonia lactiflora. The extraction rate of palmatine hydrochloride was used as the index. The optimal extraction technology of the yellow cane is as follows: six times the amount of 0.3% sulfuric acid solution soaking extract three times, each time 24 h, the extract was adjusted to pH 12, plus sodium chloride amount to salt concentration of 10%, standing 24 h; The best purification technology is as follows: crushed the crude extract of cauliflower, and 30 times of 85% ethanol was added for 30 minutes, filtered, and the ethanol was recovered to 15 times the amount, left standing for 48 hours, filtered and the residue was dried. The process can be used for the purpose of extracting and purifying Chinese cabbage herbs. The method has simple operation and lays a scientific foundation for its industrialized production and application.