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目的 研究 FN6 - 8片段能否促进损伤神经元的突起生长 .方法 从包含有 TN- C分子中 FN6 - 8DNA序列的质粒中表达并纯化 GST- FN6 - 8融合蛋白 ,以 0 .0 5 m g·L- 1的浓度加入培养的胚胎小鼠脊髓神经元的培养液中 ,对照组加入等量 GST,然后液体石蜡封闭液面造成神经元缺气损
Objective To study whether FN6 - 8 could promote neurite outgrowth.Methods GST - FN6 - 8 fusion protein was expressed and purified from plasmid containing FN6 - 8 DNA sequence of TN - C molecule at 0. 05 mg · The concentration of L-1 was added to the culture medium of embryonic mouse spinal cord neurons. The control group was given the same amount of GST, and then liquid paraffin-blocked liquid surface caused neuronal loss of air loss