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纳米金在分子生物学、生物传感器等领域具有广阔的应用前景.针对纳米金在电解质溶液中易形成不可逆聚集的问题,通过紫外光谱、TEM、Zeta电位测试等表征,研究了DNA分子修饰对纳米金溶胶稳定性的影响.结果表明,所制备的纳米金粒子的初始Zeta电位与粒径有关,平均粒径为13nm的金粒子对应-44.4mV的电位;当加入钠离子缓冲液后,纳米金粒子迅速聚集沉积.紫外光谱的动力学特性曲线表明,经过与巯基相连的DNA修饰后,纳米金粒子能够在钠离子缓冲液中稳定存在.
Nano-gold has broad application prospects in molecular biology, biosensors and other fields.Aiming at the problem that nano-gold tends to form irreversible aggregation in electrolyte solution, the effects of DNA molecule modification on the properties of nano- The results showed that the initial Zeta potential of the prepared gold nanoparticles was related to the particle size, and the gold particles with the average particle size of 13 nm corresponded to a potential of -44.4 mV. When the sodium ion buffer was added, the gold nanoparticles The particles rapidly accumulate and sediment.The dynamic characteristic curve of UV spectrum shows that the gold nanoparticles can stably exist in sodium ion buffer after DNA modification linked with sulfhydryl group.