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以换锦花(Lycoris sprengeri)为材料,采用RT-PCR方法和RACE技术相结合的方法,从花瓣中克隆了MYB基因的c DNA全长序列,命名为Ls MYB4。该序列全长793 bp,包含606 bp完整开放阅读框,编码201个氨基酸,具有明显的R2R3-MYB结构域,与中国水仙Nt MYB相似性达96%。实时荧光定量PCR分析结果表明,Ls MYB4在换锦花不同组织和不同花期均有表达,其中在花瓣中的相对表达量比叶中多9~10倍,在盛花期的相对表达量比花苞期多20倍。不同花色无性系花瓣中表达量存在差异,花色较浅的无性系高于其它无性系,推测Ls MYB4在调控换锦花花色形成的过程中起着负调控作用。
Using Lycoris sprengeri as a material, the full length cDNA of MYB gene was cloned from petal by RT-PCR and RACE techniques and named as Ls MYB4. The full length of this sequence was 793 bp and contained a 606 bp complete open reading frame (ORF) encoding 201 amino acids with an obvious R2R3-MYB domain, which was 96% similar to Nt MYB in China. The results of real-time PCR showed that Ls MYB4 was expressed in different tissues and different flowering stage of Jinhua, and the relative expression level in petals was 9-10 times more than that in leaves. The relative expression of Ls MYB4 in flowering stage was higher than that in flowering stage 20 times more. The expression of Ls MYB4 in different floral clones was different from that in other floral clones. Ls MYB4 played a negative regulatory role in the process of floral color control.