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目的:应用可注射性纤维蛋白胶(fibrin glue,FG)液态支架材料联合人胎盘间充质干细胞(placent mesen-chymal stem cells,PMSCs)进行心肌内注射,观察其对细胞滞留、存活的影响,探讨其作为可注射性心肌组织工程支架的可行性。方法:(1)分离、培养、扩增人PMSCs备用;(2)建立大鼠急性心肌梗死模型并注射FG,观察其在心肌梗死区域存留及降解情况;(3)大鼠急性心肌梗死模型随机分为4组,对照组(PBS组)、FG组、PMSCs+PBS组、PMSCs+FG组,对移植24h后细胞滞留、4周后细胞的存活及局部新生血管的密度进行测量。结果:(1)FG组织相容性好,心肌内降解时间1周左右。(2)PMSCs+FG组细胞24h滞留率显著增高,4周存活细胞数量显著增高、心肌梗死区域新生血管密度显著提高(P<0.01)。结论:可注射性纤维蛋白胶可以提高移植细胞在心肌梗死部位的滞留及存活,发挥微环境调控作用并刺激心肌梗死部位微血管生成,是良好的可注射性心肌组织工程支架材料。
OBJECTIVE: To study the effects of injectable fibrin glue (FG) liquid scaffolds and human placental mesenchymal stem cells (PMSCs) on myocardial cell retention and survival, To explore its feasibility as an injectable myocardial tissue engineering scaffold. Methods: (1) Isolation, culture and expansion of human PMSCs for backup; (2) Establishment of rat model of acute myocardial infarction and injection of FG to observe its survival and degradation in myocardial infarction area; (3) Acute myocardial infarction model in rats was randomized The cells were divided into 4 groups: control group (PBS group), FG group, PMSCs + PBS group and PMSCs + FG group. After 24h of transplantation, the cells were retained, the survival of cells and the density of local neovascularization were measured after 4 weeks. Results: (1) FG histocompatibility, myocardial degradation time of about 1 week. (2) The retention rate of cells in PMSCs + FG group was significantly increased at 24h, the number of surviving cells at 4 weeks was significantly increased, and the neovascular density was significantly increased in myocardial infarction area (P <0.01). CONCLUSION: Injectable fibrin glue can improve the retention and survival of transplanted cells in myocardial infarction, play a role in microenvironment regulation and stimulate angiogenesis in myocardial infarction. It is a good injectable myocardial tissue engineering scaffold material.