论文部分内容阅读
在新生大鼠高体延髓－脊髓标本上，用吸附电极记录膈神经根－颈4、颈5腹根（C4v、C5v）和舌下神经根（Ⅶ）节律性放电活动（Rhythmicaldischargeactivity，RDA），并观察在对延髓微切割后的RDA变化。结果（1）新生大鼠离体延髓－脊髓标本在正常灌流条件下，可存活6～8h，持续4h可稳定记录到C4v、C5v和舌下神经根的RDA，且两者完全同步，为呼吸节律性放电（RespiratoryRDA，RRDA）。（2）从头端问尾端切割延髓（每次100μm），切至闩前500μm水平时，RRDA不变，进一步切割，至闩水平，RRDA消失；从尾端问头端切割，切至舌下神经根下缘水平，RRDA不变；从延髓背侧向腹侧水平切割，切割至背→腹一半水平时，RRDA不变，进一步向腹侧切割，RRDA逐渐消失，说明从闩水平至闩前500μm的延髓腹侧半结构在呼吸节律发生中作用重要。组织学检查证实，从闩水平至闩前500μm的腹侧半结构含面神经后核内侧区（mNRF）；结果进一步提示，mNRF是呼吸节律起源的部位。
In neonatal rat medulla oblongata-spinal cord samples, phrenic nerve root-neck 4, cervical C5v (C5v) and sublingual nerve root (Ⅶ) rhythmical discharge activity (RDA) The changes in RDA after medullary microdissection were observed. Results (1) The isolated medulla oblongata-spinal cord specimens of neonatal rats survived for 6-8 h under normal perfusion conditions, and recorded stable RDA of C4v, C5v and sublingual nerve roots for 4h, which were completely synchronized Rhythmic discharge (RespiratoryRDA, RRDA). (2) The medulla oblongata (100 μm each time) was cut from the caudal end, and the RRDA was cut to the level of 500 μm before the amputation, and further cutting was performed until the latching level was reached. The RRDA disappeared. The level of inferior nerve root, RRDA unchanged; from the dorsal to the ventral medulla oblonga, cutting back to the first half of the abdomen level, RRDA unchanged further to the ventral cut, RRDA gradually disappear, indicating the level from the latch to the latch 500μm medulla oblongal half structure plays an important role in the occurrence of respiratory rhythm. Histological examination confirmed that the mNRF was located in the medial area of the posterior nucleus (mNRF) from the level of the latch to the front of 500 μm in the ventral half of the latch. The results further suggested that mNRF is the origin of respiratory rhythm.