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磁场一直以来都被认为对骨生长具有促进作用.磁性纳米颗粒可以被看作是一个磁偶极子,因此宏观的磁性纳米颗粒组装膜也可能对附近的物体具有磁效应.本文通过层层自组装方法在聚乳酸支架表面制备了宏观γ-Fe_2O_3纳米颗粒组装膜,研究了γ-Fe_2O_3纳米颗粒组装膜对原代小鼠骨髓细胞的磁作用.原代小鼠骨髓细胞从小鼠体内新鲜提取,并在前述生物材料表面培养.定量PCR用来定量表征细胞效应,磁场的影响通过检测一种刚刚发现的磁感应蛋白来指示.结果表明,表面纳米颗粒组装可以显著增强聚合物支架的力学性质,促进细胞生长和分化.磁感应蛋白检测结果表明这是由于磁性纳米颗粒组装导致的磁效应引起的.本文用磁感应蛋白证明了磁性纳米颗粒层层自组装膜可以通过对细胞的磁效应促进干细胞的生长和分化,该磁性纳米颗粒组装膜将会促进新一代组织工程支架的研发,有可能将物理刺激效应引入到体内局部组织修复中.
Magnetic field has always been considered to promote bone growth.Magnetic nanoparticles can be regarded as a magnetic dipole, so the macro-magnetic nanoparticle assembly film may also have a magnetic effect on the nearby objects.This paper through the layers of Preparation of macro-γ-Fe_2O_3 nanoparticle assembly membrane on the surface of polylactic acid scaffolds, the magnetic effect of γ-Fe_2O_3 nanoparticle assembly membrane on primary mouse bone marrow cells was studied.Myobium cells of primary mouse were freshly extracted from mice, And cultured on the surface of the aforementioned biomaterials.Quantitative PCR was used to quantitatively characterize the cellular effect, the influence of the magnetic field being indicated by the detection of a newly discovered magnetotactic protein.The results show that surface nanoparticle assembly can significantly enhance the mechanical properties of polymer scaffolds, promote Cell growth and differentiation.The magnetic induction protein test results show that this is due to the magnetic effect caused by the assembly of magnetic nanoparticles.In this paper, magnetic induction protein was used to prove that the self-assembled magnetic nanoparticle layer can promote the growth of stem cells through the magnetic effect on the cells and Differentiation, the magnetic nanoparticle assembly membrane will promote a new generation of tissue engineering branch R & D, it is possible to introduce the physical stimulus effect to the local tissue repair.