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目的 探讨survivin在大肠腺癌发病机制中的作用及其与VEGF在肿瘤转移中的关系。方法 对 42例大肠腺癌组织用逆转录PCR方法检测survivinmRNA表达 ,免疫组织化学SP法检测VEGF蛋白表达。结果 大肠腺癌组织survivin基因表达阳性率为 5 4 8%,癌旁正常组织均无表达。survivin表达与大肠腺癌分化程度、Dukes分期明显相关 ,而与性别、淋巴结转移无相关关系。VEGF表达与大肠腺癌淋巴结转移显著相关。survivin与VEGF的表达具有一致性 ,两者同时表达的大肠腺癌更容易发生淋巴结转移。结论 survivin基因表达与大肠腺癌临床病理特征和生物学行为有密切关系 ,检测survivin的表达水平 ,对于大肠腺癌的诊断、恶性程度及预后的判断均有一定价值
Objective To investigate the role of survivin in the pathogenesis of colorectal adenocarcinoma and its relationship with VEGF in tumor metastasis. Methods The expression of survivin mRNA was detected by RT-PCR in 42 cases of colorectal adenocarcinoma. The expression of VEGF protein was detected by immunohistochemical SP method. Results The positive rate of survivin gene expression was 54.8% in colorectal adenocarcinoma and no expression in normal para-cancerous tissues. The expression of survivin was significantly correlated with the differentiation of colorectal adenocarcinoma and Dukes stage, but not with the gender and lymph node metastasis. VEGF expression was significantly associated with lymph node metastasis in colorectal adenocarcinoma. The expression of survivin and VEGF is consistent, the two simultaneously expressed colorectal adenocarcinoma is more prone to lymph node metastasis. Conclusion The expression of survivin gene is closely related to the clinicopathological features and biological behavior of colorectal adenocarcinoma. To detect the expression of survivin is of value to the diagnosis, malignancy and prognosis of colorectal adenocarcinoma