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目的 观察小分子多肽类物质心肌肽素对缺氧 再给氧损伤培养心肌细胞的保护作用。方法 建立培养心肌细胞缺氧 再给氧损伤模型 ,分别以荧光微量法和光化学扩增法检测心肌细胞内脂质过氧化反应终产物MDA的含量及细胞内SOD活性 ,线粒体脱氢酶活性用四甲基偶氮唑蓝(MTT)比色法测定。结果 心肌肽素可剂量依赖性地降低MDA的含量 ,提高SOD活性及增强受损细胞内线粒体脱氢酶活性。结论 心肌肽素对缺氧 再给氧损伤培养心肌细胞具有保护作用
Objective To observe the protective effect of cardiomyopeptidin, a small molecule peptide, on cultured cardiomyocytes induced by hypoxia and reoxygenation. Methods The model of cardiomyocyte hypoxia-reoxygenation injury was established. The content of MDA and the activity of intracellular SOD, the activity of mitochondrial dehydrogenase, in cardiomyocytes were detected by fluorescence microdialysis and photochemical method. Methyl Azacyl Blue (MTT) Colorimetric Determination. Cardiomyopeptidin reduced MDA content, increased SOD activity and increased mitochondrial dehydrogenase activity in damaged cells in a dose-dependent manner. Conclusion Cardiomyopeptidin has a protective effect on cardiomyocytes cultured with hypoxia and reoxygenation