论文部分内容阅读
目的:建立简便的索他洛尔血药浓度 HPLC测定方法.方法:色谱柱为 Nova-Pak C 18(3.9mm×150mm,4μm);流动相为乙腈-0.08 mol/L磷酸二氢钾(PH 4.6)(3:97).流速1ml/min.荧光检测激发波长Ex235 nm,发射波长 Em 310 nm.血样预处理采用高氯酸直接沉淀-二氯甲烷洗涤净化法.结果:索他洛尔血药浓度在 0.10～6.40 μg/ml范围内线性关系良好(γ=0.999 9),日内、日间精密度小于 8%.结论:方法简便可靠,可较好地满足临床治疗药物监测工作的需要.
OBJECTIVE: To establish a simple HPLC method for determination of sotalol in plasma.METHODS: Nova-Pak C 18 column (3.9 mm × 150 mm, 4 μm) was used as the mobile phase. The mobile phase was acetonitrile-0.08 mol / L potassium dihydrogen phosphate 4.6) (3:97) Flow rate 1 ml / min Fluorescence detection Ex235 nm excitation wavelength Em 310 nm Pretreatment of blood samples using perchloric acid precipitation - methylene chloride purification method Results: Sotalol blood The linearity of the drug concentration in the range of 0.10-6.40 μg / ml was good (γ = 0.999 9), and the intra-day and inter-day precision was less than 8% .Conclusion: The method is simple and reliable and can meet the needs of clinical drug monitoring.