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为探明枯草芽胞杆菌PTS-394在番茄根围的定殖能力,采用电转化法获得绿色荧光蛋白(green fluorescent protein,GFP)标记菌株PTS-GFP,构建其生长曲线,采用对峙生长法评价其室内抑菌活性,并应用抗生素平板回收结合激光扫描共聚焦显微镜观察标记菌株在番茄根围的定殖数量。结果显示:与原始菌株PTS-394相比,标记菌株PTS-GFP的生长、对青枯病菌和4种病原真菌的室内抑菌能力无明显差异。标记菌株PTS-GFP和青枯病菌菌液单独或混合处理番茄苗,灌根当天标记菌株初始菌量接近108CFU/g,处理3 d后种群数量迅速下降,约106CFU/g,随后缓慢下降,处理10d后种群数量约104CFU/g,处理30 d后,标记菌株仍然能被检测到,约20 CFU/g。表明枯草芽胞杆菌PTS-394在番茄根际土壤中具有一定的定殖能力。
To determine the colonization ability of Bacillus subtilis PTS-394 in tomato rhizosphere, green fluorescent protein (GFP) -transfected strain PTS-GFP was obtained by electroporation and its growth curve was constructed. The growth of PTS- Indoor antibacterial activity, and application of antibiotic plate recovery combined with laser scanning confocal microscopy of marker colonization of the number of strains in the tomato root circumference. The results showed that compared with the original strain PTS-394, the growth of the marker strain PTS-GFP showed no significant difference in the antibacterial activity against the bacterial wilt and four pathogenic fungi. The bacterial strains of PTS-GFP and bacterial wilt of Bacterium were treated with PTS-GFP alone or in combination. The number of initial bacteria in the labeled strain on the day of irrigation was close to 108 CFU / g. After 3 days of treatment, the population decreased rapidly to about 106 CFU / g, After 10 days, the population was about 104 CFU / g. After 30 days of treatment, the marker strains could still be detected, about 20 CFU / g. The results showed that Bacillus subtilis PTS-394 had some colonization ability in tomato rhizosphere soil.