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在实验室不同温度下每2毫升水内放150条索科的奈氏线虫(Reesimermis nielseni)感染期幼虫,半数致死时间在15℃时为5.33天,25℃时为1.79天。但对蚊类幼虫的寄生率,25℃时高于15℃。如果线虫感染期幼虫和库蚊二龄幼虫的比例超过3:1,可以得到100%的感染率。
Reversal of nysensei in 150 infective larvae per 2 ml of water was performed in 2 ml of water at different temperatures in the laboratory with a median lethal time of 5.33 days at 15 ° C and 1.79 days at 25 ° C. However, parasitic rates on mosquito larvae are higher than 15 ° C at 25 ° C. If the ratio of nematode second instar larvae to Culex pipiens larvae exceeds 3: 1, the infection rate of 100% can be obtained.