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目的探讨多西环素对卵巢癌的抗肿瘤作用及可能机制。方法采用MTT方法检测多西环素单独作用及与顺铂联合用药时对卵巢癌细胞HO8910增殖的影响;采用RT-RCR检测卵巢癌细胞HO8910中CXCR4mRNA的表达;采用蛋白质免疫印迹法检测多西环素作用后卵巢癌细胞HO8910中CXCR4表达的改变。结果较低浓度多西环素(10μg/mL)作用48h后即可抑制卵巢癌细胞HO8910的增殖活力(P<0.01),当其浓度为50μg/mL时抑制率达(70±2)%。多西环素与顺铂联合用药后对癌细胞的抑制效应大于同一浓度顺铂单独作用时,可提高癌细胞对顺铂的化疗敏感性。多西环素抑制卵巢癌细胞HO8910中CXCR4的表达。结论多西环素对卵巢癌细胞HO8910有明确的抑制增殖作用,能增加癌细胞对顺铂化疗的敏感性。SDF-1/CXCR4通路可能参与其中。
Objective To investigate the anti-tumor effect of doxycycline on ovarian cancer and its possible mechanism. Methods MTT assay was used to detect the effect of doxycycline alone and in combination with cisplatin on the proliferation of ovarian cancer cells HO8910; RT-RCR was used to detect the expression of CXCR4 mRNA in ovarian cancer HO8910 cells; Western blotting was used to detect the expression of doxepin Changes of CXCR4 Expression in Ovarian Cancer HO8910 Cells after Superoxide Dismutase. Results The proliferation of ovarian cancer cell line HO8910 was inhibited by 10 μg / mL docetaxel (P <0.01) after 48 h treatment. The inhibitory rate was 70 ± 2% when the concentration was 50 μg / mL. The combination of doxycycline and cisplatin on cancer cells inhibitory effect is greater than the same concentration of cisplatin alone can increase the sensitivity of cancer cells to cisplatin. Doxycycline inhibits the expression of CXCR4 in ovarian cancer HO8910 cells. Conclusion Doxycycline has a clear inhibitory effect on the proliferation of ovarian cancer cells HO8910, which can increase the sensitivity of cancer cells to cisplatin chemotherapy. The SDF-1 / CXCR4 pathway may be involved.