ＨＬＡＢ２７因与强直性脊椎炎（ａｎｋｙｌｏｓｉｎｇｓｐｏｎｄｙｌｉｔｉｓ，ＡＳ）具强相关性而成为临床ＡＳ的诊断与鉴别诊断的重要指标［１］。对ＨＬＡＢ２７的传统检测方法多采用血清学技术，但由于难以获得高效价的单价抗血清，易出现漏诊或假阳性。近年来本室采 HLA  B27 with ankylosing spondylitis (ankylosponsive, AS) with strong correlation has become an important diagnostic and differential diagnosis of AS clinical indicators [1]. Traditional methods of detection of HLA B27 more use of serological techniques, but difficult to obtain high-titer monovalent antiserum, prone to misdiagnosis or false positive. In recent years, this room mining